Part:BBa_K1015014

IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)

With bacteriophage λ recombination sysytem, this device insert into Escherichia coli genome. This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop.

Results of agarose gel electrophoresis


Fig.1 This is result of 2nd PCR of overlap extension PCR.
The left is molecular weight marker which is λ-hindⅢ. And middle is IRR/IRL-FLP and right is FLP-Sm.


Fig2. This is result of 3rd PCR of overlap extension PCR.
The left is molecular weight marker which is λ-hindⅢ and the right is K1015015. Second from the left, the middle and second from the right is K1015015.The right is control.


Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1156
  Illegal SpeI site found at 1474
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1156
  Illegal SpeI site found at 1474
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1156
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1156
  Illegal SpeI site found at 1474
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1156
  Illegal SpeI site found at 1474
  Illegal NgoMIV site found at 2453
  
• 1000
  COMPATIBLE WITH RFC[1000]